‪Francesca Cappellini‬ - ‪Google Scholar‬

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Gamma-H2AX foci detection is the standard method to quantify DNA double-strand break (DSB) induction and repair. In this study, we investigated the induction and decay of γ-H2AX foci of different tumor cell lines and fibroblasts with known mutations in DNA damage repair genes, including ATM, LigIV, DNA-PKcs, Rad51 and Rad54. A radiation dose of 2.4 Gy was used for either an acute single high We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry. For all chemicals the cytotoxic dose response was assayed by a metabolic cytotoxicity assay. injectors for kinetic assays. The instrument was used to image the stained DNA using the GFP imaging channel.

Mix well. Pipette 392 μL of Assay Buffer into the 800 pM tube. gamma H2AX 18 results for gamma Multiple steps standard assay (2) Assay type Clear. Semi-quantitative (2) Biologically active Clear. No (1) Detection method AMSBIO announces the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. This new assay has been developed for anti-cancer drug screening, basic research and Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.}, author = {Moquet, Jayne and Barnard, Stephen and Staynova, Albena and Lindholm, Carita and Monteiro Gil, Octávia and Martins, Vanda and Rößler, Ute and Vral, Anne Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle.

Unirradiated pe- ripheral blood 1 Feb 2013 Experimental Design: γ-H2AX foci formation was validated preclinically in comparison with the Comet assay, and evaluated 21 Aug 2012 This assay is based on the phosphorylation of H2AX histone in response to Use of the [gamma]H2AX assay for assessing the genotoxicity of The role of γ-H2AX foci in the DNA damage signaling pathway induced by For the alkaline comet assay, the comet assay method from Trevigen was used 29 Apr 2003 DSBs (Foci) Can Be Quantified After Doses as Low as 1 mGy. We next determined the sensitivity of the γ-H2AX assay with regard to the lowest IR 30 Sep 2016 Upon phosphorylation H2AX is referred to as gamma-H2AX.

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PLOS One 2013; 8: 1-12. doi: 10.1371/journal.pone. 007954116.

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Investigating γ H2AX as a Biomarker of Radiosensitivity Using Flow Cytometry subsets, CD19 in particular, might be proved to be a more sensitive assay. GV-Failover Server is a video backup server that records up to 128 IP streams from hosts GV-Systems.

95 GAMMA: 3.45k, 59.5k, 118.8k/TM119 Condition D GAMMA SOURCE: Co6 thermal neutrons, gamma-rays. K ey detector mma-H2AX assay, blood king water,. CBRN. SALIANT. Selective An tibodies Limited Immun o.
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set an ROI in one of the foci inside cell(nucleus). 2010-02-04 Asked 23rd Nov, 2017. Ariel Shimoni. I stained pancreatic cancer cells (Panc-1) for gamma-H2AX and got a large amount of foci in control cells (cells which have not undergone any genotoxic stress 2013-07-08 CONCLUSIONS: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories. PMID: 27686523 [Indexed for MEDLINE] Publication Types: Comparative Study; Evaluation Studies; Validation Studies; MeSH terms gamma-H2AX Pharmacodynamic Assay Kit. The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies.

To substantiate the similarity observed between the human and minipig we show that minipig fibroblast radiosensitivity was similar to that observed with human fibroblasts. A number of ELISA Kits are available that target gammaH2ax, an alias of the protein, H2A histone family member X. The human protein, encoded by the gene H2AFX, is a member of the Histone H2A family. The protein's cellular localization is indicated to be nuclear. Additional synonyms or past names for this protein may include: H2A.X, H2A/X, H2AX.
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Supplemental Information. P-TEFb Activation by RBM7

In such studies, this approach is applied as a biomarker of genomic instability, likely as a result of deficiencies in DNA Gamma-H2AX is used widely as DNA damage marker in vitro, but its use for genotoxicity assessment in vivo has not been extensively investigated. Here, we developed an image analysis system for the precise quantification of the gamma-H2AX signal, which we used to monitor DNA damage in animals treated with known genotoxicants (EMS, ENU and doxorubicin). Laboratories around the world now use the gamma-H2AX assay developed by Bonner to study how cells sense and respond to double-strand breaks. The CCR team also used the gamma-H2AX assay to discover that cells’ genomic integrity may decline as we age in part because aging cells assemble damage repair machinery more slowly. The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). Moquet J(1), Barnard S(1), Staynova A(2), Lindholm C(3), Monteiro Gil O(4), Martins V(4), Rößler U(5), Vral A(6), Vandevoorde C(6)(7), Wojewódzka M(8), Rothkamm K(1)(9). Over the past decade, the γ-H2AX assay has been applied to a variety of cell types and tissues to correlate γ-H2AX levels with DNA damage and repair [ 9, 10, 11, 12, 13 ].

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Thus, with γH2AX we have a bio-indicator on hand for critical DNA lesions and severe genomic replication stress. Hence, the γH2AX assay is not an assay for detecting any type of DNA damage, but for biologically relevant and severe DNA damage (including DNA interstrand crosslinks that give rise to DSBs; Nikolova et al., 2012). Phosphorylated H2AX (gamma-H2AX) is essential to the efficient recognition and (or) repair of DNA double strand breaks (DSBs), and many molecules, often thousands, of H2AX become rapidly phosphorylated at the site of each nascent DSB. An antibody to gamma-H2AX reveals that this highly amplified process generates nuclear foci.

The gamma-H2AX assay has up to now not been applied in this context, and it is a promising tool for investigating the early cellular response to mixed beam irradiation.